5′-RACEing Across a Bridging Oligonucleotide
نویسندگان
چکیده
منابع مشابه
5'-RACEing across a bridging oligonucleotide.
Rapid amplification of cDNA 5′ ends (5′-RACE) is a convenient way to retrieve information about the 5′ termini of mRNAs because entire open reading frames are difficult to predict from genomic information and expressed sequence tag libraries generally lack full 5′ termini. To achieve this, numerous methods have been developed, each of which has been successful in specific situations (3–5,11–14,...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 2002
ISSN: 0736-6205,1940-9818
DOI: 10.2144/02323bm01